Description
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 17-OHCS concentrations in Human serum, plasma and other body fluids. Using Purified Human 17-OHCS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 17-OHCS and 17-OHCS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 17-OHCS in the samples is then determined by comparing the O.D. of the samples to the standard curve